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Rhizosphere metagenome analysis and wet-lab approach to derive optimal strategy for lead remediation in situ

Bhat et al. | Jul 18, 2023

Rhizosphere metagenome analysis and wet-lab approach to derive optimal strategy for lead remediation <i>in situ</i>
Image credit: Karolina Grabowska

The Environmental Protection Agency (EPA) reports a significant number of heavy metal-contaminated sites across the United States. To address this public health concern, rhizoremediation using microbes has emerged as a promising solution. Here, a combination of soil microbes were inoculated in the rhizosphere in soil contaminated with 500 parts per million (ppm) of lead. Results showed rhizoremediation is an effective bioremediation strategy and may increase crop productivity by converting nonarable lands into arable lands.

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Phages Can Be More Effective and Specific Than Antibiotics in Combating Bacteria

Wu et al. | Feb 17, 2019

Phages Can Be More Effective and Specific Than Antibiotics in Combating Bacteria

Phage therapy has been suggested as an alternative to antibiotics because bacteria resistant to antibiotics may still be susceptible to phages. However, phages may have limited effectiveness in combating bacteria since bacteria possess several antiviral defense mechanisms and can quickly develop resistance to phages. In this study, Wu and Pinta compare the effectiveness and specificity of antibiotics and phages in combating bacteria. They found that T4 phages are more specific and effective in fighting or inhibiting both antibiotic-resistant and sensitive bacteria than antibiotics, suggesting that phage therapy can be developed as an efficient tool to combat antibiotic-resistant bacteria.

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The effects of Helianthus Annuus on Amyotrophic Lateral Sclerosis using Drosophila Melanogaster

Srinivasan et al. | Oct 13, 2022

The effects of <em>Helianthus Annuus</em> on Amyotrophic Lateral Sclerosis using <em>Drosophila Melanogaster</em>

Amyotrophic lateral sclerosis (ALS) affects nearly 200,000 people worldwide and there is currently no cure. The purpose of the study was to determine if Helianthus annuus seeds helped reduce nerve degeneration and increase locomotion using Drosophila melanogaster as the model organism. Through this experiment, we found a general trend suggesting that H. annuus helped increase the mobility of the D. melanogaster suggesting it could be a viable supplement for patients with ALS.

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Impact of simple vs complex carbohydrates under time constraint before anaerobic and aerobic exercise

Cui et al. | Oct 13, 2022

Impact of simple vs complex carbohydrates under time constraint before anaerobic and aerobic exercise

The goal of this study was to determine the if carbohydrates or complex carbohydrates are better for athlete's performance in anaerobic and aerobic exercise. Ultimately, we found that, when one’s schedule only allows for 30 minutes to eat before a workout, the best pre-workout meal for optimal glycogen levels to prompt muscle hypertrophy, strength increases, and better endurance is one that is simple carbohydrate-heavy.

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The role of xpa-1 and him-1 in UV protection of Caenorhabditis elegans

Tung et al. | Feb 25, 2022

The role of <em>xpa-1</em> and <em>him-1</em> in UV protection of <em>Caenorhabditis elegans</em>

Caenorhabditis elegans xpa-1 and him-1 are orthologs of human XPA and human SMC1A, respectively. Mutations in the XPA are correlated with Xeroderma pigmentosum, a condition that induces hypersensitivity to ultraviolet (UV) radiation. Alternatively, SMC1A mutations may lead to Cornelia de Lange Syndrome, a multi-organ disorder that makes patients more sensitive to UVinduced DNA damage. Both C. elegans genes have been found to be involved in protection against UV radiation, but their combined effects have not been tested when they are both knocked down. The authors hypothesized that because these genes are involved in separate pathways, the simultaneous knockdown of both of these genes using RNA interference (RNAi) in C. elegans will cause them to become more sensitive to UV radiation than either of them knocked down individually. UV protection was measured via the percent survival of C. elegans post 365 nm and 5.4x10-19 joules of UV radiation. The double xpa-1/him-1 RNAi knockdown showed a significantly reduced percent survival after 15 and 30 minutes of UV radiation relative to wild-type and xpa-1 and him-1 single knockdowns. These measurements were consistent with their hypothesis and demonstrated that xpa-1 and him-1 genes play distinct roles in resistance against UV stress in C. elegans. This result raises the possibility that the xpa-1/him-1 double knockdown could be useful as an animal model for studying the human disease Xeroderma pigmentosum and Cornelia de Lange Syndrome.

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