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Increased carmine red exposure periods yields a higher number of vacuoles formed in Tetrahymena pyriformis

Shah et al. | Nov 18, 2022

Increased carmine red exposure periods yields a higher number of vacuoles formed in <em>Tetrahymena pyriformis</em>

T. pyriformis can use phagocytosis to create vacuoles of carmine red, a dye which is made using crushed insects and is full of nutrients. Establishing a relationship between vacuole formation and duration of exposure to food can demonstrate how phagocytosis occurs in T. pyriformis. We hypothesized that if T. pyriformis was incubated in a carmine red solution, then more vacuoles would form over time in each cell.

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Application of gene therapy for reversing T-cell dysfunction in cancer

Hyun Lee et al. | Aug 25, 2022

Application of gene therapy for reversing T-cell dysfunction in cancer

Since cancer cells inhibit T-cell activity, the authors investigated a method to reverse T-cell disfunction with gene therapy, so that the T-cells would become effective once again in fighting cancer cells. They used the inhibition of proprotein convertases (PCSK1) in T cells and programmed death-ligand 1 (CD274) in cancer cells. They observed the recovery of IL-2 expression in Jurkat cells, with increased recovery noted in a co-culture sample. This study suggests a novel strategy to reactivate T cells.

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Effect Of SMC On The Growth Of Bean, Cherry Tomato And Roma Tomato Plant

Rao et al. | Sep 12, 2020

Effect Of SMC On The Growth Of Bean, Cherry Tomato And Roma Tomato Plant

Mushroom compost, also called Spent Mushroom Substrate or Spent Mushroom Compost (SMC), is suitable for a variety of plants. Previous research has found that the application of SMC will increase plant growth. However, it is unclear which exact proportions of SMC and soil will maximize tomato and bean plant growth. We showed that the hypothesized growth media with 30% SMC optimizes seed germination, plant height, number of leaves, and survival rate compared to other combinations of growth media. Our research suggests that SMC is a useful alternative for conventional fertilizers.

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Presoaking Seeds with Vinegar Improves Seed Development and Drought Tolerance in Maize Plants

D'Agate et al. | Jul 24, 2020

Presoaking Seeds with Vinegar Improves Seed Development and Drought Tolerance in Maize Plants

Climate change has contributed to the increasing annual temperatures around the world and poses a grave threat to Maize crops. Two methods proven to help combat plant drought stress effects are presoaking seeds (seeds are soaked in a liquid before planting) and the application of Acetic Acid (vinegar) to soil. The purpose of this experiment was to explore if combining these two methods by presoaking seeds with a vinegar solution can improve the seed development and plant drought tolerance of Maize plants during drought conditions.

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POC-MON: A Novel and Cost-Effective Pocket Lemon Sniff Test (PLST) for Early Detection of Major Depressive Disorder

Cruz et al. | Jul 07, 2020

POC-MON: A Novel and Cost-Effective Pocket Lemon Sniff Test (PLST) for Early Detection of Major Depressive Disorder

Effective treatment of depression requires early detection. Depressive symptoms overlap with olfactory regions, which led to several studies of the correlation between sense of smell and depression. The alarming rise of depression, its related crimes, suicides, and lack of inexpensive, quick tools in detecting early depression — this study aims in demonstrating decreased olfaction and depression correlation. Forty-two subjects (ages 13-83) underwent POC-MON (Pocket Lemon) assessment — an oven-dried lemon peel sniff test, subjected to distance measurement when odor first detected (threshold) and completed Patient Health Questionnaires (PHQ-9). POC-MON and PHQ-9 scores yielded a correlation of 20% and 18% for the right and left nostrils, respectively. Among male (n=17) subjects, the average distance of POC-MON and PHQ-9 scores produced a correlation of 14% and 16% for the right and left nostrils, respectively. Females (n=25) demonstrated a correlation of 28% and 21% for the right and left nostrils, respectively. These results suggest the correlation between olfaction and depression in diagnosing its early-stage, using a quick, inexpensive, and patient-friendly tool — POC-MON.

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