One important factor that contributes to human cancers is accumulated damage to cells' DNA due to the oxidative stress caused by free radicals. In this study, the authors investigate the effects of several different tea leaf extracts on oxidative stress in cultured human prostate cells to see if antioxidants in the tea leaves could help protect cells from this type of DNA damage. They found that all four types of tea extract (as well as direct application of the antioxidant EGCG) improved the outcomes for the cultured cells, with white tea extract having the strongest effect. This research suggests that tea extracts and the antioxidants that they contain may have applications in the treatment of the many diseases associated with cellular DNA damage, including cancer.
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QuitPuff: A Simple Method Using Saliva to Assess the Risk of Oral Pre-Cancerous Lesions and Oral Squamous Cell Carcinoma in Chronic Smokers
Smoking generates free radicals and reactive oxygen species which induce cell damage and lipid peroxidation. This is linked to the development of oral cancer in chronic smokers. The authors of this study developed Quitpuff, simple colorimetric test to measure the extent of lipid peroxidation in saliva samples. This test detected salivary lipid peroxidation with 96% accuracy in test subjects and could serve as an inexpensive, non-invasive test for smokers to measure degree of salivary lipid peroxidation and potential risk of oral cancer.
Read More...The external presence of running water influences the root growth of pea plants (Phaselous vulgaris)
Each year, invasive tree roots cause large amounts of damage to underground pipes. While this is usually due to leaks and cracks, tree roots can also invade pipes that are structurally sound. We are interested in investigating whether plant roots have an affinity towards flowing water, measured through mass, even when the running water is not in direct contact with soil. We tested this by creating a choice chamber with water running under one end and no stimulus on the other end. Overall, the masses of the roots growing towards flowing water were greater than the masses of the roots growing towards the end with no stimulus, showing that plant roots did have an affinity towards flowing water.
Read More...Assessing the Efficacy of NOX Enzyme Inhibitors as Potential Treatments for Ischemic Stroke in silico
Ischemic stroke occurs when blood flow to the brain is interrupted, causing brain damage. This study investigated the effectiveness of different NOX inhibitors as treatments for ischemic stroke in silico. The results help corroborate previous in vivo and in vitro studies in an in silico format, and can be used towards developing drugs to treat ischemic stroke.
Read More...Identification of microwave-related changes in tissue using an ultrasound scan
Microwave energy (ME) is used in the medical field to denature protein structures, resulting in inactivation or destruction of abnormal cells. Identifying the extent of destruction of abnormal tissue (cancer tissue or tissue with abnormal electrical activity) is essential for accomplishing successful therapy and reducing collateral damage. Our study was an ex vivo assessment of the changes on ultrasound scans (US) in chicken tissue exposed to ME. We hypothesized that any changes in tissue structures would be recognized on the reflected ultrasound waves. Ultrasound scans of tissues change with exposure to microwaves with increasing reflection of ultrasound waves. With exposure to microwaves, surface level brightness on the ultrasound scans increases statistically significantly. The findings could be used in heat related (ME and radiofrequency) procedures where clinicians would be able to actively assess lesions in real-time. Further studies are required to assess changes in tissue during active exposure to different types of energies.
Read More...The effect of nicotine and lead on neuron morphology, function, and ɑ-Synuclein levels in a C. elegans model
E-cigarettes are often considered a healthier alternative to traditional cigarettes. This team of high school authors investigated the impact of common e-cigarette compounds on C. elegans, and found a number of harmful effects ultimately resulting in injury and neuronal damage.
Read More...Phytochemical Analysis of Amaranthus spinosus Linn.: An in vitro Analysis
Mainstream cancer treatments, which include radiotherapy and chemotherapeutic drugs, are known to induce oxidative damage to healthy somatic cells due to the liberation of harmful free radicals. In order to avert this, physiological antioxidants must be complemented with external antioxidants. Here the authors performed a preliminary phytochemical screen to identify alkaloids, saponins, flavonoids, polyphenols, and tannins in all parts of the Amaranthus spinosus Linn. plant. This paper describes the preparation of this crude extract and assesses its antioxidant properties for potential use in complementary cancer treatment.
Read More...The Development of a Superhydrophobic Surface Using Electrolytic Deposition & Polymer Chains Precipitation
In this study, the authors were interested in developing a hydrophobic surface that will extend the lifespan of metals by reducing water exposure and other damage. The used a zinc coating on steel to pursue this effort.
Read More...The Effect of Common Cations on DNA Degradation
Heating of DNA-containing solutions is a part of many experiment protocols, but it can also cause damage and degradation of the DNA molecules, potentially leading to error in the experimental results. The authors of this paper investigate whether the presence of certain cations during heating can stabilize the DNA polymer and aid the preservation of the molecule.
Read More...The Effect of Antioxidant Vitamins on Mustard Plants in a Hydrogen Peroxide-Induced Injury Model
In this study, the authors assess the antioxidant properties of vitamins A, C and E given to mustard plants after oxidative damage. This research shows an interesting comparison of the vitamins' effect on plant recovery and health.
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