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Optimizing data augmentation to improve machine learning accuracy on endemic frog calls

Anand et al. | Mar 09, 2025

Optimizing data augmentation to improve machine learning accuracy on endemic frog calls
Image credit: Anand and Sampath 2025

The mountain chain of the Western Ghats on the Indian peninsula, a UNESCO World Heritage site, is home to about 200 frog species, 89 of which are endemic. Distinctive to each frog species, their vocalizations can be used for species recognition. Manually surveying frogs at night during the rain in elephant and big cat forests is difficult, so being able to autonomously record ambient soundscapes and identify species is essential. An effective machine learning (ML) species classifier requires substantial training data from this area. The goal of this study was to assess data augmentation techniques on a dataset of frog vocalizations from this region, which has a minimal number of audio recordings per species. Consequently, enhancing an ML model’s performance with limited data is necessary. We analyzed the effects of four data augmentation techniques (Time Shifting, Noise Injection, Spectral Augmentation, and Test-Time Augmentation) individually and their combined effect on the frog vocalization data and the public environmental sounds dataset (ESC-50). The effect of combined data augmentation techniques improved the model's relative accuracy as the size of the dataset decreased. The combination of all four techniques improved the ML model’s classification accuracy on the frog calls dataset by 94%. This study established a data augmentation approach to maximize the classification accuracy with sparse data of frog call recordings, thereby creating a possibility to build a real-world automated field frog species identifier system. Such a system can significantly help in the conservation of frog species in this vital biodiversity hotspot.

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An exploration of western mosquitofish as the animal component in an aquaponic farming system

Medina et al. | Dec 03, 2024

An exploration of western mosquitofish as the animal component in an aquaponic farming system
Image credit: The authors

Aquaponics (the combination of aquatic plant farming with fish production) is an innovative farming practice, but the fish that are typically used, like tilapia, are expensive and space-consuming to cultivate. Medina and Alvarez explore other options test if mosquitofish are a viable option in the aquaponic cultivation of herbs and microgreens.

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Optimizing surface contact area and electrolyte type to develop a more effective rechargeable battery

Rajapakse et al. | Oct 27, 2024

Optimizing surface contact area and electrolyte type to develop a more effective rechargeable battery
Image credit: Rajapakse and Rajapakse 2024.

Rechargeable batteries are playing an increasingly prominent role in our lives due to the ongoing transition from fossil energy sources to green energy. The purpose of this study was to investigate variables that impact the effectiveness of rechargeable batteries. Alkaline (non-rechargeable) and rechargeable batteries share common features that are critical for the operation of a battery. The positive and negative electrodes, also known as the cathode and anode, are where the energy of the battery is stored. The electrolyte is what facilitates the transfer of cations and anions in a battery to generate electricity. Due to the importance of these components, we felt that a systematic investigation examining the surface area of the cathode and anode as well the impact of electrolytes with different properties on battery performance was justified. Utilizing a copper cathode and aluminum anode coupled with a water in salt electrolyte, a model rechargeable battery system was developed to test two hypotheses: a) increasing the contact area between the electrodes and electrolyte would improve battery capacity, and b) more soluble salt-based electrolytes would improve battery capacity. After soaking in an electrolyte solution, the battery was charged and the capacity, starting voltage, and ending voltage of each battery were measured. The results of this study supported our hypothesis that larger anode/cathodes surface areas and more ionic electrolytes such as sodium chloride, potassium chloride and potassium sulfate resulted in superior battery capacity. Incorporating these findings can help maximize the efficiency of commercial rechargeable batteries.

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Effect of Gram-positive bacteria on antibiotic resistance in Gram-negative bacteria

Willem et al. | Mar 12, 2024

Effect of Gram-positive bacteria on antibiotic resistance in Gram-negative bacteria

Antibiotics are one of the most common treatments for bacterial infections, but the emergence of antibiotic resistance is a major threat to the control of infectious diseases. Many factors contribute to the development of antibiotic resistance. One is bacterial conjugation from Gram-positive to Gram-negative bacteria where there is a transfer of resistance genes from Gram-positive to Gram-negative bacteria that could increase antibiotic resistance in the latter. In light of these observations, we decided to test whether Gram-negative bacteria that came into contact with Gram-positive bacteria had a higher resistance to the antimicrobial properties of spices than Gram-negative bacteria that did not come into contact with Gram-positive bacteria.

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Reduce the harm of acid rain to plants by producing nitrogen fertilizer through neutralization

Xu et al. | Apr 25, 2023

Reduce the harm of acid rain to plants by producing nitrogen fertilizer through neutralization
Image credit: Ave Calvar Martinez, pexels.com

The phenomenon of dying trees and plants in areas affected by acid rain has become increasingly problematic in recent times. Is there any method to efficiently utilize the rainwater and reduce the harmfulness of acid rain or make it beneficial to plants? This study aimed to investigate the potential of neutralizing acid rainwater infiltrating the soil to increase soil pH, produce beneficial salts for plants, and support better plant growth. To test this hypothesis, precipitation samples were collected from six states in the U.S. in 2022, and the pH of the acid rain was measured to obtain a representative pH value for the country. Experiments were then conducted to simulate the neutralization of acid rain and the subsequent change in soil pH levels. To evaluate the effectiveness and feasibility of this method, cat grass was planted in pots of soil soaked with solutions mimicking acid rain, with control and experimental groups receiving neutralizing agents (ammonium hydroxide) or not. Plant growth was measured by analyzing the height of the plants. Results demonstrated that neutralizing agents were effective in improving soil pH levels and that the resulting salts produced were beneficial to the growth of the grass. The findings suggest that this method could be applied on a larger agricultural scale to reduce the harmful effects of acid rain and increase agricultural efficiency.

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Association of agenesis of the corpus callosum with epilepsy and anticonvulsant drug treatment

Steger et al. | Feb 21, 2023

Association of agenesis of the corpus callosum with epilepsy and anticonvulsant drug treatment
Image credit: Robina Weermeijer on Unsplash

Agenesis of the Corpus Callosum (ACC) is a birth defect where an infant’s corpus callosum, the structure linking the brain’s two hemispheres to allow interhemispheric communication, fails to develop in a typical manner during pregnancy. Existing research on the connection between ACC and epilepsy leaves significant gaps, due to the lack of focused investigation. One important gap is the degree to which ACC may impact the course of epilepsy treatment and outcomes. The present study was conducted to test the hypotheses that epilepsy is highly prevalent among individuals with ACC, and that those with both ACC and epilepsy have a lower response rate to anticonvulsant drugs than other patients treated with anticonvulsant drugs. A weighted average of epilepsy rates was calculated from a review of existing literature, which supported the hypothesis that epilepsy was more common among individuals with ACC (25.11%) than in the general population (1.2%). An empirical survey administered to 57 subjects or parents of subjects showed that rate of intractable epilepsy among study subjects with both ACC and epilepsy was substantially higher than the rate found in the general population, indicating that individuals with both conditions had a lower response rate to the anticonvulsant drugs. This study contributes novel results regarding the potential for concurrence of ACC and epilepsy to interfere with anticonvulsant drug treatment. We also discuss implications for how medical professionals may use the findings of this study to add depth to their treatment decisions.

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Reading recall: A comparison of reading comprehension

Rudins et al. | Nov 16, 2022

Reading recall: A comparison of reading comprehension

Researchers query whether reading comprehension is the same, worse, or better when using e-books as compared with standard paper texts. This study evaluated this question in the elementary school population. Our hypothesis was that information would be retained equally whether read from paper or from an electronic device. Each participant read four stories, alternating between electronic and paper media types. After each reading, the participants completed a five-question test covering the information read. The study participants correctly answered 167 out of 200 comprehension questions when reading from an electronic device. These same participants correctly answered 145 out of 200 comprehension questions when reading from paper. At a significance level of p < 0.05, the results showed that there was a statistically significant difference in reading comprehension between the two media, demonstrating better comprehension when using electronic media. The unexpected results of this study demonstrate a shift in children’s performance and desirability of using electronic media as a reading source.

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Contrasting role of ASCC3 and ALKBH3 in determining genomic alterations in Glioblastoma Multiforme

Sriram et al. | Sep 27, 2022

Contrasting role of <i>ASCC3</i> and <i>ALKBH3</i> in determining genomic alterations in Glioblastoma Multiforme

Glioblastoma Multiforme (GBM) is the most malignant brain tumor with the highest fraction of genome alterations (FGA), manifesting poor disease-free status (DFS) and overall survival (OS). We explored The Cancer Genome Atlas (TCGA) and cBioportal public dataset- Firehose legacy GBM to study DNA repair genes Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) and Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 3 (ALKBH3). To test our hypothesis that these genes have correlations with FGA and can better determine prognosis and survival, we sorted the dataset to arrive at 254 patients. Analyzing using RStudio, both ASCC3 and ALKBH3 demonstrated hypomethylation in 82.3% and 61.8% of patients, respectively. Interestingly, low mRNA expression was observed in both these genes. We further conducted correlation tests between both methylation and mRNA expression of these genes with FGA. ASCC3 was found to be negatively correlated, while ALKBH3 was found to be positively correlated, potentially indicating contrasting dysregulation of these two genes. Prognostic analysis showed the following: ASCC3 hypomethylation is significant with DFS and high ASCC3 mRNA expression to be significant with OS, demonstrating ASCC3’s potential as disease prediction marker.

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Identifying Neural Networks that Implement a Simple Spatial Concept

Zirvi et al. | Sep 13, 2022

Identifying Neural Networks that Implement a Simple Spatial Concept

Modern artificial neural networks have been remarkably successful in various applications, from speech recognition to computer vision. However, it remains less clear whether they can implement abstract concepts, which are essential to generalization and understanding. To address this problem, the authors investigated the above vs. below task, a simple concept-based task that honeybees can solve, using a conventional neural network. They found that networks achieved 100% test accuracy when a visual target was presented below a black bar, however only 50% test accuracy when a visual target was presented below a reference shape.

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Antimicrobial properties of common household spices on microbes cultured from two kitchen locations

Carroll et al. | May 12, 2022

Antimicrobial properties of common household spices on microbes cultured from two kitchen locations

The number of bacterial infections in humans is rising, and a major contributor is foodborne illnesses, which affect a large portion of the population and result in many hospitalizations and deaths. Common household cleaners are an effective strategy to combat foodborne illness, but they are often costly and contain harmful chemicals. Thus, the authors sought to test the antimicrobial effectiveness of spices (clove, nutmeg, astragalus, cinnamon, turmeric, and garlic) on microbes cultured from refrigerator handles and cutting boards. Results from this study demonstrate long-lasting, antimicrobial effects of multiple spices that support their use as alternatives to common household cleaners.

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