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Fluorescein or Green Fluorescent Protein: Is It Possible to Create a Sensor for Dehydration?

Joshi et al. | Dec 09, 2019

Fluorescein or Green Fluorescent Protein: Is It Possible to Create a Sensor for Dehydration?

Currently there is no early dehydration detection system using temperature and pH as indicators. A sensor could alert the wearer and others of low hydration levels, which would normally be difficult to catch prior to more serious complications resulting from dehydration. In this study, a protein fluorophore, green fluorescent protein (GFP), and a chemical fluorophore, fluorescein, were tested for a change in fluorescence in response to increased temperature or decreased pH. Reversing the pH change did not restore GFP fluorescence, but that of fluorescein was re-established. This finding suggests that fluorescein could be used as a reusable sensor for a dehydration-related pH change.

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The optimization of high-protein duckweed cultivation in eutrophicated water with mutualistic bacteria

Akkarajeerawat et al. | Mar 18, 2025

The optimization of high-protein duckweed cultivation in eutrophicated water with mutualistic bacteria

he rapid growth of the human population is driving food crises in Thailand and Southeast Asia, while contributing to global food insecurity and a larger carbon footprint. One potential solution is cultivating duckweed (Wolffia globosa) for consumption, as it grows quickly and can provide an alternative protein source. This research explored two methods to optimize duckweed cultivation: using phosphorus- and nitrogen-rich growing media and plant growth-promoting bacteria (PGPB).

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