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In this article, the authors analyzed ribosome profiling data from amino acid-starved pancreatic cancer cells to explore whether the pattern of ribosome distribution along transcripts under normal conditions can predict the degree of ribosome stalling under stress. The authors found that ribosomes in amino acid-deprived cells stalled more along elongation-limited transcripts. By contrast, they observed no relationship between read density near start and stop and disparities between mRNA sequencing reads and ribosome profiling reads. This research identifies an important relationship between read distribution and propensity for ribosomes to stall, although more work is needed to fully understand the patterns of ribosome distribution along transcripts in ribosome profiling data.

This study aimed to obtain an optimal non-antibiotic method to suppress the growth of pathogenic bacteria within the body. The two-fold purpose of this project was to determine which combination of bacteria would result in the most biofilm formation and then to assess the effect of ayurvedic plant extracts on the biofilm. The results show that the addition of a plant extract can affect the biofilm growth of a bacteria combination. The applications of this study can be used to design probiotic supplements with added beneficial plant extracts.

This study tested the proficiency of different concentrations of the antimicrobial sorbic acid to inhibit the probiotic Lactobacillus casei Shirota. It was hypothesized that sorbic acid’s use as a bacterial deterrent would also target this bacterial strain of Lactobacillus. The results supported the hypothesis, with the colony count of L. casei Shirota having significant decreases at all concentrations of sorbic acid. These results additionally suggest that even under the FDA sorbic acid restrictions of 0.03% concentration, damaging effects could be seen in L. casei Shirota.

Human amylase is important to digestion and has broad applications for therapeutic use in patients with pancreatic insufficiency. The authors present a method to increase amylase production in E. coli by adding the amino acids L-glutamate and L-glutamine.

Recognizing the increasing threat of acid deposition inn soil through the reaction of NOx and SO₂ pollutants with water in Spain, the authors investigates the effects of Al(NO₃)₃ concentrations on the health of Allium sativum. By tracking its mitotic index, they found a negative exponential correlation between Al(NO₃)₃ concentrations and the mitotic index of A. sativum.

Climate change has contributed to the increasing annual temperatures around the world and poses a grave threat to Maize crops. Two methods proven to help combat plant drought stress effects are presoaking seeds (seeds are soaked in a liquid before planting) and the application of Acetic Acid (vinegar) to soil. The purpose of this experiment was to explore if combining these two methods by presoaking seeds with a vinegar solution can improve the seed development and plant drought tolerance of Maize plants during drought conditions.

Saccharomyces cerevisiae yeast is used to produce bioethanol, an alternative to fossil fuels. In this study, authors take advantage of this well studied yeast by genetically engineering them to increase fatty acid biosynthesis and culturing in a cost-effective wastewater based medium; potentially providing a sustainable alternative to petrochemicals.

In organic synthesis, protecting groups are derivatives of reactive functionalities that play a key role in ensuring chemoselectivity of chemical transformations. To protect alcohols and amines, acid-labile tert-butyloxycarbonyl protecting groups are often employed but are avoided when the substrate is acid-sensitive. Thus, orthogonal base-labile protecting groups have been in demand to enable selective deprotection and to preserve the reactivity of acid-sensitive substrates. To meet this demand, we present 4-nitrophenyl carbonates and carbamates as orthogonal base-labile protecting group strategies.

In this study, the authors design a series of new biaryl small molecules to target and block the binding pocket of the enzyme dihydropteroate synthase, which is important for prokaryotic biosynthesis of folic acid and could serve as better antimicrobial compounds.

In this article, Harvell and Nicholson hypothesized that increased ocean acidity would decrease the photosynthetic ability of Chaetoceros gracilis, a diatom prolific in Monterey Bay, because of the usually corrosive effects of carbonic acid on both seashells and cells’ internal structures. They altered pH of algae environments and measured the photosynthetic ability of diatoms over four days by spectrophotometer. Overall, their findings indicate that C. gracilis may become more abundant in Monterey Bay as the pH of the ocean continues to drop, potentially contributing to harmful algal blooms.