In this article, Harvell and Nicholson hypothesized that increased ocean acidity would decrease the photosynthetic ability of Chaetoceros gracilis, a diatom prolific in Monterey Bay, because of the usually corrosive effects of carbonic acid on both seashells and cells’ internal structures. They altered pH of algae environments and measured the photosynthetic ability of diatoms over four days by spectrophotometer. Overall, their findings indicate that C. gracilis may become more abundant in Monterey Bay as the pH of the ocean continues to drop, potentially contributing to harmful algal blooms.
Read More...Browse Articles
Effects of various alkaline carbonic solutions on the growth of the freshwater algae Chlorophyceae
Modern day fossil fuels are prone to polluting our environment, which can provide major habitat loss to many animals in our ecosystems. Algae-based biofuels have become an increasingly popular alternative to fossil fuels because of their sustainability, effectiveness, and environmentally-friendly nature. To encourage algae growth and solidify its role as an emerging biofuel, we tested basic (in terms of pH) solutions on pond water to determine which solution is most efficient in inducing the growth of algae.
Read More...Taft linear free-energy relationships in the biocatalytic hydrolysis of sterically hindered nitrophenyl ester substrates
This study applies Taft linear free-energy relationships to study kinetic trends in the enzymatic hydrolysis of sterically hindered substrates.
Read More...The Inhibitory Effect of Probiotics on the Growth and Biofilm Formation of Salmonella Sp.
Salmonella is a genus of bacteria responsible for over 90 million cases of intestinal illnesses yearly. Like many bacteria, Salmonella can create a biofilm matrix, which confers stronger resistance against antibiotics. However, there has been relatively little research on the inhibition of Salmonella biofilm formation, which is a crucial factor in its widespread growth. In this study, Lee and Kim quantitatively measure the effectiveness of several common probiotics in inhibiting Salmonella bacterial growth. They found concentration-dependent antibacterial effects varied among the probiotics tested, indicating the possibility of probiotic species-specific mechanisms of Salmonella growth inhibition.
Read More...Biofortification of Raphanus sativus through irrigation with Ca2+ solutions does not increase calcium content
This study is centered around developing biofortification methods: the authors test whether the amount of calcium available to growing crops translates into more calcium present in the crops.
Read More...Spectrophotometric comparison of 4-Nitrophenyl carbonates & carbamates as base-labile protecting groups
In organic synthesis, protecting groups are derivatives of reactive functionalities that play a key role in ensuring chemoselectivity of chemical transformations. To protect alcohols and amines, acid-labile tert-butyloxycarbonyl protecting groups are often employed but are avoided when the substrate is acid-sensitive. Thus, orthogonal base-labile protecting groups have been in demand to enable selective deprotection and to preserve the reactivity of acid-sensitive substrates. To meet this demand, we present 4-nitrophenyl carbonates and carbamates as orthogonal base-labile protecting group strategies.
Read More...Mechanistic deconvolution of autoreduction in tetrazolium-based cell viability assays
Optical reporters like tetrazolium dyes, exemplified by 5-diphenyl tetrazolium bromide (MTT), are effective tools for quantifying cellular responses under experimental conditions. These dyes assess cell viability by producing brightly-colored formazan dyes when reduced inside active cells. However, certain small molecules, including reducing agents like ascorbic acid, cysteine, and glutathione (GSH), can interfere with MTT assays, potentially compromising accuracy.
Read More...The Effects of Micro-Algae Characteristics on the Bioremediation Rate of Deepwater Horizon Crude Oil
Environmental disasters such as the Deepwater Horizon oil spill can be devastating to ecosystems for long periods of time. Safer, cheaper, and more effective methods of oil clean-up are needed to clean up oil spills in the future. Here, the authors investigate the ability of natural ocean algae to process crude oil into less toxic chemicals. They identify Coccochloris elabens as a particularly promising algae for future bioremediation efforts.
Read More...Utilizing a Wastewater-Based Medium for Engineered Saccharomyces cerevisiae for the Biological Production of Fatty Alcohols and Carboxylic Acids to Replace Petrochemicals
Saccharomyces cerevisiae yeast is used to produce bioethanol, an alternative to fossil fuels. In this study, authors take advantage of this well studied yeast by genetically engineering them to increase fatty acid biosynthesis and culturing in a cost-effective wastewater based medium; potentially providing a sustainable alternative to petrochemicals.
Read More...