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Comparative singlet oxygen photosensitizer efficiency of berberine, rose bengal, and methylene blue by time course nuclear magnetic resonance (NMR) monitoring of a photochemical 4+2 cycloaddition endoperoxide formation

Su et al. | May 14, 2021

Comparative singlet oxygen photosensitizer efficiency of berberine, rose bengal, and methylene blue by time course nuclear magnetic resonance (NMR) monitoring of a photochemical 4+2 cycloaddition endoperoxide formation

Berberine, a natural product alkaloid, has been shown to exert biological activity via in situ production of singlet oxygen when photo irradiated. Berberine utilizes singlet oxygen in its putative mechanism of action, wherein it forms an activated complex with DNA and photosensitizes triplet oxygen to singlet oxygen to specifically oxidize guanine residues, thereby halting cell replication and leading to cell death. This has potential application in photodynamic therapy, alongside other such compounds which also act as photosensitizers and produce singlet oxygen in situ. The quantification of singlet oxygen in various photosensitizers, including berberine, is essential for determining their photosensitizer efficiencies. We postulated that the singlet oxygen produced by photoirradiation of berberine would be superior in terms of singlet oxygen production to the aforementioned photosensitizers when irradiated with UV light, but inferior under visible light conditions, due to its strong absorbance of UV wavelengths.

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The Effect of Ultraviolet Radiation and the Antioxidant Curcumin on the Longevity, Fertility, and Physical Structure of Drosophila melanogaster: Can We Defend Our DNA?

Lateef et al. | May 18, 2019

The Effect of Ultraviolet Radiation and the Antioxidant Curcumin on the Longevity, Fertility, and Physical Structure of <em>Drosophila melanogaster</em>: Can We Defend Our DNA?

Ultraviolet (UV) radiation is known to alter DNA structure and impair cellular function in all living organisms. In this study, Lateef et al examine the effects of UV radiation to determine whether antioxidant-enriched nutrition can combat the potential deleterious effects of UV radiation on Drosophila melanogaster. They found that UVB (320nm) radiation caused a 59% decrease in the Drosophila lifespan and mutagenic effects on flies' physical appearance, but did not significantly affect fertility. Curcumin significantly prolonged lifespan and enhanced fertility for both UV- and non-UV-exposed flies. The research demonstrates the positive potential of natural antioxidants as weapons against radiation-induced diseases including cancer.

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Evaluation of platelet-rich plasma vs. platelet lysate: VEGF and PDGF concentration, stability, and shelf life

Prasad et al. | Mar 30, 2022

Evaluation of platelet-rich plasma vs. platelet lysate: VEGF and PDGF concentration, stability, and shelf life

Cell-free biologicals are a novel method of treating clinical conditions which involve chronic inflammation such as tendonitis and osteoarthritis. This study compared platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) in platelet-rich plasma (PRP), activated PRP (aPRP), and platelet lysate (PL). It was hypothesized that PL would contain higher concentrations of growth factors than PRP and that different storage temperatures for PL would diminish cytokine expression. Results demonstrated PL had the highest concentrations of both cytokines, with concentrations slightly diminishing at-80C. aPRP and PRP demonstrated lower concentrations of PDGF and VEGF than PL.

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RNAi-based Gene Therapy Targeting ZGPAT Promotes EGF-dependent Wound Healing

Lee et al. | Nov 15, 2021

RNAi-based Gene Therapy Targeting ZGPAT Promotes EGF-dependent Wound Healing

Wound-healing involves a sequence of events, such as inflammation, proliferation, and migration of different cell types like fibroblasts. Zinc Finger CCCH-type with G-Patch Domain Containing Protein (ZGPAT), encodes a protein that has its main role as a transcription repressor by binding to a specific DNA sequence. The aim of the study was to find out whether inhibiting ZGPAT will expedite the wound healing process by accelerating cell migration. This treatment strategy can provide a key to the development of wound healing strategies in medicine and cellular biology.

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Comparative Gamma Radiation Analysis by Geographic Region

Zadan et al. | Jul 20, 2015

Comparative Gamma Radiation Analysis by Geographic Region

Gamma radiation can be produced by both natural and man-made sources and abnormally high exposure levels could lead to an increase in cell damage. In this study, gamma radiation was measured at different locations and any correlation with various geographic factors, such as distance from a city center, elevation and proximity to the nearest nuclear reactor, was determined.

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Identification of a Free Radical Scavenger as an Additive for Lung Transplant Preservation Solution to Inhibit Coagulative Necrosis and Extend Organ Preservation

Ganesh et al. | Feb 12, 2015

Identification of a Free Radical Scavenger as an Additive for Lung Transplant Preservation Solution to Inhibit Coagulative Necrosis and Extend Organ Preservation

During transfer of organs from a donor to a patient, the organs deteriorate in part due to damage by free radicals. Application of antioxidant solutions could extend organ preservation times. The authors found that vitamin E and butylated hydroxytoluene seemed to be most effective in arresting cell damage of a bovine lung.

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Investigating KNOX Gene Expression in Aquilegia Petal Spur Development

Hossain et al. | Feb 03, 2014

Investigating KNOX Gene Expression in Aquilegia Petal Spur Development

Plants, and all other multi-cellular organisms, develop through the coordinated action of many sets of genes. The authors here investigate the genes, in a class named KNOX, potentially responsible for organizing a certain part of Aquilegia (columbine) flowers called petal spurs. Through the technique Reverse Transcription-Polymerase Chain Reaction (RT-PCR), they find that certain KNOX genes are expressed non-uniformly in petal spurs, suggesting that they may be involved, perhaps in a cell-specific manner. This research will help guide future efforts toward understanding how many beautiful flowers develop their unique shapes.

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