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The authors describe the design and testing of new guide RNAs targeting the DMPK gene, which is responsible for myotonic dystrophy.

In this article the authors look at the lung microbiome in patients with cystic fibrosis to determine what the major bacterial species present are.

Major Depressive Disorder (MDD), and Post-Traumatic Stress Disorder (PTSD) are two of the fastest growing comorbid diseases in the world. Using publicly available datasets from the National Institute for Biotechnology Information (NCBI), Ravi and Lee conducted a differential gene expression analysis using 184 blood samples from either control individuals or individuals with comorbid MDD and PTSD. As a result, the authors identified 253 highly differentially-expressed genes, with enrichment for proteins in the gene ontology group 'Ribosomal Pathway'. These genes may be used as blood-based biomarkers for susceptibility to MDD or PTSD, and to tailor treatments within a personalized medicine regime.

India accounts for over 2.4 million recorded cases of tuberculosis, about 26% of the world’s cases. This research ascertained the bearing of both the population density and the average elevation above mean sea level (MSL) on the number of cases of TB recorded by the districts in Maharashtra, India. The results found a strong positive correlation between the number of TB cases per thousand people and the population density and a strong negative correlation between the number of TB cases per thousand people and the average elevation above MSL.

The misfolding of proteins leads to numerous diseases including Akzheimer’s, Parkinson’s and Type II Diabetes. Understanding of exactly how proteins fold is crucial for many medical advancements. Chenna and Englander addressed this problem by measuring the rate of hydrogen-deuterium exchange within proteins exposed to deuterium oxide in order to further elucidate the process of protein folding. Here, mass spectrometry was used to measure exchange in Cytochrome c and was compared to archived 1H NMR data.

Antibiotics are used to treat dangerous diseases. Over time, however, bacteria are becoming resistant to antibiotics - which poses a threat to humans and animals alike. In this paper, the authors examine how E. coli gains resistance to the antibiotic amoxicillin.

Systematic consumption of traditional foods is a popular way of treating diseases in India. Rasam, a soup of spices and tomato with a tamarind base, is a home remedy for viral infections such as the common cold. Here, we investigate if rasam, prepared under household conditions, exhibits antibacterial activity against Escherichia coli and Staphylococcus aureus, two common pathogenic bacteria. Our results show rasam prepared under household conditions lacks antibacterial activity despite its ingredients possessing such properties.

Cutibacterium acnes is a bacterium believed to play an important role in the pathogenesis of common skin diseases such as acne vulgaris. Currently, acne is known to be associated with strains from the type IA1 and IC clades of C. acnes, while those from the type IA2, IB, II, and III phylogroups are associated with skin health. This is the first study to explore the sequence space of individual gene products of different C. acnes phylogroups. Our analysis compared the sequence space topology of virulence factors to proteins with unknown functions and housekeeping proteins. We hypothesized that sequence space features of virulence factors are different from housekeeping protein features, which potentially provides an avenue to deduce unknown proteins’ functions. This proposition should be confirmed based on further experimental outcomes. A notable similarity in the sequence spaces’ topological features of previously known as housekeeping proteins encoded by recA and guaA genes to ‘putative virulence’ genes camp2 and tly was observed. Our research suggests further investigation of recA and guaA’s potential virulence properties to better understand acne pathogenesis and develop more targeted acne treatments.

The emergence of antibiotic-resistant pathogenic bacteria is a major concern for human health, rendering some antibiotics ineffective in treating diseases. The authors of this study tested the hypothesis that exposing rumen bacteria to tetracycline will gradually lead to the development of tetracycline-resistant bacteria, some of which will develop multidrug resistance.

The study explored the role of testosterone beyond its well-established effects on male sex characteristics, focusing on its association with non-communicable diseases (NCDs) like obesity and type 2 diabetes (T2D), using Mendelian randomization (MR) analysis on genomic data.