Human cytomegalovirus (HCMV) causes serious infections in immunocompromised patients and therapies to inhibit latent HCMV are not developed. Using CRISPR/Cas9, the authors were able to delete an important promoter region in HCMV.
Read More...Using CRISPR technology to inhibit the replication of human cytomegalovirus by deletion of a gene promoter
Human cytomegalovirus (HCMV) causes serious infections in immunocompromised patients and therapies to inhibit latent HCMV are not developed. Using CRISPR/Cas9, the authors were able to delete an important promoter region in HCMV.
Read More...Aggression of Carcharhinus leucas and Carcharhinus amblyrhynchos towards humans
This paper presents findings on Carcharhinus leucas (bull shark) and Carcharhinus amblyrhynchos (grey reef shark) aggression towards humans at Beqa Adventure Divers in Shark Reef Marine Reserve, Fiji. We hypothesized that grey reef sharks would receive more prods than bull sharks because grey reef sharks are typically more aggressive than bull sharks. The results supported our hypothesis, as an individual grey reef shark received 2.44 prods on average per feed, while a bull shark had an average of 0.61. These findings are meaningful not only to the world’s general understanding of shark aggression, but also to human protection against grey reef sharks as well as public education on bull sharks and the conservation of the species.
Read More...The Effect of the Human MeCP2 gene on Drosophila melanogaster behavior and p53 inhibition as a model for Rett Syndrome
In this study, the authors observe if the symptoms of Rett Syndrome, a neurodegenerative disease in humans, are reflected in Drosophila melanogaster. This was achieved by differentiating the behavior and physical aspects of wild-type flies from flies expressing the full-length MeCP2 gene and the mutated MeCP2 gene (R106W). After conducting these experiments, some of the Rett Syndrome symptoms were recapitulated in Drosophila, and a subset of those were partially ameliorated by the introduction of pifithrin-alpha.
Read More...Simulations of Cheetah Roaming Demonstrate the Effect of Safety Corridors on Genetic Diversity and Human-Cheetah Conflict
Ecological corridors are geographic features designated to allow the movement of wildlife populations between habitats that have been fragmented by human landscapes. Corridors can be a pivotal aspect in wildlife conservation because they preserve a suitable habitat for isolated populations to live and intermingle. Here, two students simulate the effect of introducing a safety corridor for cheetahs, based on real tracking data on cheetahs in Namibia.
Read More...Estimation of cytokines in PHA-activated mononuclear cells isolated from human peripheral and cord blood
In this study, the authors investigated the time-dependent cytokine secretion ability of phyto-hemagglutinin (PHA)-activated T cells derived from human peripheral (PB) and cord blood (CB). They hypothesized that the anti-inflammatory cytokine, IL-10, and pro-inflammatory cytokine, TNFα, levels would be higher in PHA-activated T cells obtained from PB as compared to the levels obtained from CB and would decrease over time. Upon PHA-activation, the IL-10 levels were relatively high while the TNFα levels decreased, making these findings applicable in therapeutic treatments e.g., rheumatoid arthritis, psoriasis, and organ transplantation.
Read More...A comparison study in the expansion of human bone marrow mesenchymal stem cells
In this study, the effects of different sources of serum on growing mesenchymal stem cells are compared with the goal of identifying one more suitable for clinical use.
Read More...How has California’s Shelter-in-Place Order due to COVID-19 and the Resulting Reduction in Human Activity Affected Air and Water Quality?
As the world struggled to grapple with the emerging COVID-19 pandemic in 2020, many countries instated policies to help minimize the spread of the virus among residents. This inadvertently led to a decrease in travel, and in some cases, industrial output, two major sources of pollutants in today's world. Here, the authors investigate whether California's shelter-in-place policy was associated with a measurable decrease in water and air pollution in that state between June and July of 2020, compared to the preceeding five years. Their findings suggest that, by some metrics, air quality improved within certain areas while water quality was relatively unchanged. Overall, these findings suggest that changing human behavior can, indeed, help reduce the level of air pollutants that compromise air quality.
Read More...Protective effect of bromelain and pineapple extracts on UV-induced damage in human skin cells
In this study, the authors tested whether the compound bromelain extracted from pineapples could protect skin cells from UV damage.
Read More...Using Graphene Oxide to Efficiently Filter Particulate Matter at High Concentrations
Air pollution has detrimental effects on both the environment and humans. Here, researchers use graphene oxide to filter particulate matter from the air. Graphene oxide filters performed better than commercially available filters, effectively removing particulate matter from the air.
Read More...The non-nutritive sweeteners acesulfame potassium and neotame slow the regeneration rate of planaria
The consumption of sugar substitute non-nutritive sweeteners (NNS) has dramatically increased in recent years. Despite being advertised as a healthy alternative, NNS have been linked to adverse effects on the body, such as neurodegenerative diseases (NDs). In NDs, neural stem cell function is impaired, which inhibits neuron regeneration. The purpose of this study was to determine if the NNS acesulfame potassium (Ace-K) and neotame affect planaria neuron regeneration rates. Since human neurons may regenerate, planaria, organisms with extensive regenerative capabilities due to stem cells called neoblasts, were used as the model organism. The heads of planaria exposed to either a control or non-toxic concentrations of NNS were amputated. The posterior regions of the planaria were observed every 24 hours to see the following regeneration stages: (1) wound healing, (2) blastema development, (3) growth, and (4) differentiation. The authors hypothesized that exposure to the NNS would slow planaria regeneration rates. The time it took for the planaria in the Ace-K group and the neotame group to reach the second, third, and fourth regeneration stage was significantly greater than that of the control. The results of this study indicated that exposure to the NNS significantly slowed regeneration rates in planaria. This suggests that the NNS may adversely impact neoblast proliferation rates in planaria, implying that it could impair neural stem cell proliferation in humans, which plays a role in NDs. This study may provide insight into the connection between NNS, human neuron regeneration, and NDs.
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