Formulation of novel polyherbal compound MAT20 with phytochemicals found in amla, tulsi, and moringa

Herbal products play an important role in mitigating the harmful effects of oxidative stress caused by free radicals. The purpose of this study was to prepare a novel antioxidant polyherbal formulation as a supplemental treatment to reduce oxidative stress. We hypothesized that the formulation “MAT20”, a polyherbal mixture containing three herbal plants, Phyllanthus emblica (amla), Ocimum sanctum (tulsi), and Moringa oleifera (moringa), possesses maximum antioxidant properties compared to each individual herb. These herbs are known in Ayurveda for their medicinal use. After obtaining extracts by using five different solvents (water, methanol, ethanol, chloroform, and acetone), we tested each for polyphenols, flavonoids, alkaloids, tannins, and saponins, which are known to have antioxidant properties and free radical scavenging activity against a free radical which reduces in the presence of antioxidants. After extensive analysis, we formulated a combination of the herbs by extracting moringa with chloroform, amla with acetone, and tulsi with methanol, which we named “MAT20." Our results show that “MAT20”, a polyherbal formulation, is an excellent source of antioxidants.