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Characterizing Quorum Sensing-Induced Bioluminescence in Variable Volumes With Vibrio fischeri Using Computer Processing Methods

Abdel-Azim et al. | Jun 22, 2020

Characterizing Quorum Sensing-Induced Bioluminescence in Variable Volumes With <em>Vibrio fischeri</em> Using Computer Processing Methods

Understanding how bacteria respond to other bacteria could facilitate their ability to initiate and maintain their infectiousness. The phenomenon by which bacteria signal to each other via chemical signals is called quorum sensing, which could be targeted to deter bacterial infection in some cases if better understood. In this article, the authors study how a bacterium called V. fischeri uses quorum sensing to change bioluminescence, an easy readout that facilitates studying quorum sensing in this strain.

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Leaky expression of PRMT1 in Escherichia coli and redox regulation of its histone methyltransferase activity

Kim et al. | Oct 07, 2026

Leaky expression of PRMT1 in <i>Escherichia coli</i> and redox regulation of its histone methyltransferase activity

This study characterizes recombinant human Protein arginine methyltransferase 1 (PRMT1) expressed in Escherichia. coli and reveals that the enzyme exhibits leaky expression even without isopropyl β-D-1-thiogalactopyranoside (IPTG) induction. Through in vitro histone methyltransferase assays, we demonstrate that PRMT1 activity toward histone H4R3 is enhanced by dithiothreitol (DTT) in a dose-dependent manner but remains unresponsive to β-mercaptoethanol. These findings highlight the critical role of redox conditions in regulating PRMT1 catalytic function and provide a practical framework for future biochemical and kinetic studies.

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Development of novel biodegradable bioplastics for packaging film using mango peels

Wang et al. | Apr 06, 2025

Development of novel biodegradable bioplastics for packaging film using mango peels
Image credit: JACQUELINE BRANDWAYN

Here the authors explored the development of biodegradable bioplastic films derived from mango peels as a sustainable solution to plastic pollution and greenhouse gas emissions from fruit waste. They optimized the film's mechanical properties and water resistance through adjusting processing conditions and incorporating plasticizers and a hydrophobic coating, ultimately demonstrating its potential as a bacteriostatic and biodegradable alternative to conventional plastic food wrap.

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