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Chronic alcohol consumption can cause cardiac myopathy, which afflicts about 500,000 Americans annually. Gunturi et al. wanted to understand the effects of alcohol on heart rate and confirm the role of nitric oxide (NO) signaling in heart rate regulation. Using the model organism Daphnia magna, a water crustacean with a large, transparent heart, they found that the heart rate of Daphnia magna was reduced after treatment with alcohol. This depression could be reversed after treatment with inhibitors of NO synthesis and signaling. Their work has important implications for how we understand alcohol-induced effects on heart rate and potential treatments to reverse heart rate depression as a result of alcohol consumption.

This study applies Taft linear free-energy relationships to study kinetic trends in the enzymatic hydrolysis of sterically hindered substrates.

As the world moves towards more eco-friendly methods for chemical synthesis, there's a strong interest in employing enzymes in chemical synthetic processes. Here, the authors explore how the activity of enzymes such as trypsin, lipase and nattokinase is affected by the electronic effects of the substrate they are acting on.

Cell-free biologicals are a novel method of treating clinical conditions which involve chronic inflammation such as tendonitis and osteoarthritis. This study compared platelet-derived growth factor (PDGF) and vascular endothelial growth factor (VEGF) in platelet-rich plasma (PRP), activated PRP (aPRP), and platelet lysate (PL). It was hypothesized that PL would contain higher concentrations of growth factors than PRP and that different storage temperatures for PL would diminish cytokine expression. Results demonstrated PL had the highest concentrations of both cytokines, with concentrations slightly diminishing at-80C. aPRP and PRP demonstrated lower concentrations of PDGF and VEGF than PL.

For public health, drinking water should be free of bacterial contamination. The objective of this research is to identify the fate of bacteria if drinking water becomes contaminated and inform consumers on which water type enables the least bacteria to survive. We hypothesized that bottled mineral water would provide the most sufficient conditions for E. coli to survive. We found that if water becomes contaminated, the conditions offered by the three water types at room temperature allow E. coli to survive up to three days. At 72 hours, the bottled spring water had the highest average colony forming units (CFUs), with tap and mineral water CFU values statistically lower than spring water but not significantly different from each other. The findings of this research highlight the need of implementing accessible quality drinking water for the underserved population and for the regulation of water sources.

Glioblastoma Multiforme (GBM) is the most malignant brain tumor with the highest fraction of genome alterations (FGA), manifesting poor disease-free status (DFS) and overall survival (OS). We explored The Cancer Genome Atlas (TCGA) and cBioportal public dataset- Firehose legacy GBM to study DNA repair genes Activating Signal Cointegrator 1 Complex Subunit 3 (ASCC3) and Alpha-Ketoglutarate-Dependent Dioxygenase AlkB Homolog 3 (ALKBH3). To test our hypothesis that these genes have correlations with FGA and can better determine prognosis and survival, we sorted the dataset to arrive at 254 patients. Analyzing using RStudio, both ASCC3 and ALKBH3 demonstrated hypomethylation in 82.3% and 61.8% of patients, respectively. Interestingly, low mRNA expression was observed in both these genes. We further conducted correlation tests between both methylation and mRNA expression of these genes with FGA. ASCC3 was found to be negatively correlated, while ALKBH3 was found to be positively correlated, potentially indicating contrasting dysregulation of these two genes. Prognostic analysis showed the following: ASCC3 hypomethylation is significant with DFS and high ASCC3 mRNA expression to be significant with OS, demonstrating ASCC3’s potential as disease prediction marker.

In this article, the authors investigate the RNA expression differences between groups of chronic cocaine abusers and drug-free subjects.

Chronic bad breath, or halitosis, is a problem faced by nearly 50% of the general poluation, but existing treatments such as liquid mouthwash or sugar-free gum are imperfect and temporary solutions. In this study, the authors investigate potential alternative treatments using natural ingredients such as Manuka Honey and Licorice root extract. They found that Manuka honey is almost as effective as commercial mouthwashes in reducing the growth of P gingivalis (one of the main bacteria that causes bad breath), while Licorice root extract was largely ineffective. The authors' results suggest that Manuka honey is a promising candidate in the search for new and improved halitosis treatments.

Here, the authors recognized the tendency of bacteria to form biofilms, where this behavior offers protection against threats such as antibiotics. To investigate this, they observed the effects of sublethal exposure of the antibiotic ampicillin on E. coli biofilm formation with an optical density crystal violet assay. They found that exposure to ampicillin resulted in the favored formation of biofilms over time, as free-floating bacteria were eradicated.

Berberine, a natural product alkaloid, and its analogs have a wide range of medicinal properties, including antibacterial and anticancer effects. Here, the authors explored a library of alkyl or aryl berberine analogs to probe binding to double-stranded and G-quadruplex DNA. They determined that the nature of the substituent, the position of the substituent, and the nucleic acid target affect the free energy of binding of berberine analogs to DNA and G-quadruplex DNA, however berberine analogs did not result in net stabilization of G-quadruplex DNA.